Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.
Identifieur interne : 000158 ( Main/Exploration ); précédent : 000157; suivant : 000159Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.
Auteurs : Thu V. Vuong [Canada] ; Bing Liu [Suède] ; Mats Sandgren [Suède] ; Emma R. Master [Canada]Source :
- Biomacromolecules [ 1526-4602 ] ; 2017.
Descripteurs français
- KwdFr :
- Cellulose (composition chimique), Chitine (composition chimique), Fluorescence (MeSH), Microtechnologie (méthodes), Mixed function oxygenases (métabolisme), Oxydoréduction (MeSH), Phanerochaete (croissance et développement), Phanerochaete (enzymologie), Polyosides (composition chimique), Spectroscopie photoélectronique (MeSH), Spécificité du substrat (MeSH).
- MESH :
- composition chimique : Cellulose, Chitine, Polyosides.
- croissance et développement : Phanerochaete.
- enzymologie : Phanerochaete.
- métabolisme : Mixed function oxygenases.
- méthodes : Microtechnologie.
- Fluorescence, Oxydoréduction, Spectroscopie photoélectronique, Spécificité du substrat.
English descriptors
- KwdEn :
- Cellulose (chemistry), Chitin (chemistry), Fluorescence (MeSH), Microtechnology (methods), Mixed Function Oxygenases (metabolism), Oxidation-Reduction (MeSH), Phanerochaete (enzymology), Phanerochaete (growth & development), Photoelectron Spectroscopy (MeSH), Polysaccharides (chemistry), Substrate Specificity (MeSH).
- MESH :
- chemical , chemistry : Cellulose, Chitin, Polysaccharides.
- chemical , metabolism : Mixed Function Oxygenases.
- enzymology : Phanerochaete.
- growth & development : Phanerochaete.
- methods : Microtechnology.
- Fluorescence, Oxidation-Reduction, Photoelectron Spectroscopy, Substrate Specificity.
Abstract
Most existing methods for screening the activity of lytic polysaccharide mono-oxygenases (LPMOs) on polysaccharides are based on the detection of soluble oxidized sugars. This approach might underestimate the total performance of LPMOs since oxidation events that do not lead to oligosaccharide release are not detected. Using PcLPMO9D as a model enzyme, a microplate-based method has been developed to detect C1-oxidizing LPMO activity by covalently linking a water-soluble fluorophore to oxidized positions within the cellulose fiber. This fluorescence method was validated using X-ray photoelectron spectroscopy and then combined with high-performance anion-exchange chromatography to track total PcLPMO9D activity.
DOI: 10.1021/acs.biomac.6b01790
PubMed: 28125213
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.</title><author><name sortKey="Vuong, Thu V" sort="Vuong, Thu V" uniqKey="Vuong T" first="Thu V" last="Vuong">Thu V. Vuong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5</wicri:regionArea><wicri:noRegion>Ontario M5S 3E5</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Bing" sort="Liu, Bing" uniqKey="Liu B" first="Bing" last="Liu">Bing Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala</wicri:regionArea><wicri:noRegion>750 07 Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Sandgren, Mats" sort="Sandgren, Mats" uniqKey="Sandgren M" first="Mats" last="Sandgren">Mats Sandgren</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala</wicri:regionArea><wicri:noRegion>750 07 Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Master, Emma R" sort="Master, Emma R" uniqKey="Master E" first="Emma R" last="Master">Emma R. Master</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5</wicri:regionArea><wicri:noRegion>Ontario M5S 3E5</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28125213</idno><idno type="pmid">28125213</idno><idno type="doi">10.1021/acs.biomac.6b01790</idno><idno type="wicri:Area/Main/Corpus">000174</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000174</idno><idno type="wicri:Area/Main/Curation">000174</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000174</idno><idno type="wicri:Area/Main/Exploration">000174</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.</title><author><name sortKey="Vuong, Thu V" sort="Vuong, Thu V" uniqKey="Vuong T" first="Thu V" last="Vuong">Thu V. Vuong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5</wicri:regionArea><wicri:noRegion>Ontario M5S 3E5</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Bing" sort="Liu, Bing" uniqKey="Liu B" first="Bing" last="Liu">Bing Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala</wicri:regionArea><wicri:noRegion>750 07 Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Sandgren, Mats" sort="Sandgren, Mats" uniqKey="Sandgren M" first="Mats" last="Sandgren">Mats Sandgren</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala</wicri:regionArea><wicri:noRegion>750 07 Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Master, Emma R" sort="Master, Emma R" uniqKey="Master E" first="Emma R" last="Master">Emma R. Master</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5</wicri:regionArea><wicri:noRegion>Ontario M5S 3E5</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biomacromolecules</title><idno type="eISSN">1526-4602</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (chemistry)</term><term>Chitin (chemistry)</term><term>Fluorescence (MeSH)</term><term>Microtechnology (methods)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (growth & development)</term><term>Photoelectron Spectroscopy (MeSH)</term><term>Polysaccharides (chemistry)</term><term>Substrate Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellulose (composition chimique)</term><term>Chitine (composition chimique)</term><term>Fluorescence (MeSH)</term><term>Microtechnologie (méthodes)</term><term>Mixed function oxygenases (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (enzymologie)</term><term>Polyosides (composition chimique)</term><term>Spectroscopie photoélectronique (MeSH)</term><term>Spécificité du substrat (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Chitin</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Chitine</term><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microtechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mixed function oxygenases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microtechnologie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescence</term><term>Oxidation-Reduction</term><term>Photoelectron Spectroscopy</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fluorescence</term><term>Oxydoréduction</term><term>Spectroscopie photoélectronique</term><term>Spécificité du substrat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most existing methods for screening the activity of lytic polysaccharide mono-oxygenases (LPMOs) on polysaccharides are based on the detection of soluble oxidized sugars. This approach might underestimate the total performance of LPMOs since oxidation events that do not lead to oligosaccharide release are not detected. Using PcLPMO9D as a model enzyme, a microplate-based method has been developed to detect C1-oxidizing LPMO activity by covalently linking a water-soluble fluorophore to oxidized positions within the cellulose fiber. This fluorescence method was validated using X-ray photoelectron spectroscopy and then combined with high-performance anion-exchange chromatography to track total PcLPMO9D activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28125213</PMID><DateCompleted><Year>2017</Year><Month>10</Month><Day>11</Day></DateCompleted><DateRevised><Year>2017</Year><Month>12</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1526-4602</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>02</Month><Day>13</Day></PubDate></JournalIssue><Title>Biomacromolecules</Title><ISOAbbreviation>Biomacromolecules</ISOAbbreviation></Journal><ArticleTitle>Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.</ArticleTitle><Pagination><MedlinePgn>610-616</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.biomac.6b01790</ELocationID><Abstract><AbstractText>Most existing methods for screening the activity of lytic polysaccharide mono-oxygenases (LPMOs) on polysaccharides are based on the detection of soluble oxidized sugars. This approach might underestimate the total performance of LPMOs since oxidation events that do not lead to oligosaccharide release are not detected. Using PcLPMO9D as a model enzyme, a microplate-based method has been developed to detect C1-oxidizing LPMO activity by covalently linking a water-soluble fluorophore to oxidized positions within the cellulose fiber. This fluorescence method was validated using X-ray photoelectron spectroscopy and then combined with high-performance anion-exchange chromatography to track total PcLPMO9D activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vuong</LastName><ForeName>Thu V</ForeName><Initials>TV</Initials><Identifier Source="ORCID">0000-0002-4753-6975</Identifier><AffiliationInfo><Affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Bing</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sandgren</LastName><ForeName>Mats</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Molecular Sciences, Swedish University of Agricultural Sciences , 750 07 Uppsala, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Master</LastName><ForeName>Emma R</ForeName><Initials>ER</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto , 200 College Street, Toronto, Ontario M5S 3E5, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biomacromolecules</MedlineTA><NlmUniqueID>100892849</NlmUniqueID><ISSNLinking>1525-7797</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>1398-61-4</RegistryNumber><NameOfSubstance UI="D002686">Chitin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002686" MajorTopicYN="N">Chitin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="Y">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055616" MajorTopicYN="N">Microtechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056951" MajorTopicYN="N">Photoelectron Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>10</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28125213</ArticleId><ArticleId IdType="doi">10.1021/acs.biomac.6b01790</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>Suède</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Vuong, Thu V" sort="Vuong, Thu V" uniqKey="Vuong T" first="Thu V" last="Vuong">Thu V. Vuong</name></noRegion><name sortKey="Master, Emma R" sort="Master, Emma R" uniqKey="Master E" first="Emma R" last="Master">Emma R. Master</name></country><country name="Suède"><noRegion><name sortKey="Liu, Bing" sort="Liu, Bing" uniqKey="Liu B" first="Bing" last="Liu">Bing Liu</name></noRegion><name sortKey="Sandgren, Mats" sort="Sandgren, Mats" uniqKey="Sandgren M" first="Mats" last="Sandgren">Mats Sandgren</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000158 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000158 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28125213
|texte= Microplate-Based Detection of Lytic Polysaccharide Monooxygenase Activity by Fluorescence-Labeling of Insoluble Oxidized Products.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28125213" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |